Jenny, Yvonne, Anne, Catherine and Tabitha were playing for a rolling jackpot of £11,000.
They said they were feeling confident having won their last quiz at The Brown Bear.
After four rounds on the randomly chosen subjects of music, science, history and sport they were neck and neck with the teams having won two rounds each. However, they were narrowly defeated in the final general knowledge round.
Team spokesperson Jenny told host Jeremy Vine that she was a relative newcomer to Berwick having decided to move here after passing on the train. Jeremy agreed the area was beautiful.