The hugely popular programme, which has been running since 1979, will film an episode of its 44th season at the former colliery site, near Ashington, with host, Fiona Bruce.
The date of the recording and the broadcast date of the episode will be announced soon.
Rowan Brown, chief executive of Museums Northumberland, said: “As a life-long Antiques Roadshow fan, I'm delighted that Woodhorn Museum will be the series' first coalmining location.
“We're really looking forward to working with the Antiques Roadshow Team to showcase Northumberland's heritage and create opportunities for our communities to get involved.
“The programme is renowned for its engaging and diverse storytelling, and Museums Northumberland aspires to do the same, so I hope we can learn a lot, and have a great time in the process.”
The episode at Woodhorn Museum will be filmed on a closed set with an invited audience to ensure the safety of guests, production and the wider public during the coronavirus pandemic.
Members of the public interested in taking part in the recording at Woodhorn Museum can apply online by visiting bbc.co.uk/antiquesroadshow.
Successful applicants will be invited to Woodhorn Museum later this summer where they will be able to share their items with a Roadshow expert who will reveal more about the craftsmanship, history and provenance of each piece – as well as the all-important value.
Returning for her 14th year as presenter, Fiona Bruce has acknowledged the team’s success at recording the show under challenging circumstances in 2020, and is encouraging people to apply to be a part of the programme in 2021.
Fiona said: “We were thrilled that we managed to make a series of the Antiques Roadshow last summer, despite all the difficulties of filming during the pandemic.
“Hopefully things will be easier this summer though life may not yet be back to normal by the time we start filming. So if you have an item that you’d like to bring along to the Roadshow, do please get in touch with us beforehand. Here’s hoping for a summer of great finds!”